|
KMID : 0881720140290040316
|
|
Journal of Food Hygiene and Safety
2014 Volume.29 No. 4 p.316 ~ p.321
|
|
|
Virulence Factor Profiles of Escherichia coli O157:H7 Bacteriophage Isolates from Sewage and Livestock Stools
|
|
Seo Ji-Na
Seo Dong-Joo
Lee Min-Hwa
Jeon Su-Been
Oh Hye-Jin
Oh Mi-Hwa
Choi Chang-Sun
|
|
|
Abstract
|
|
|
|
The aim of study was to investigate the virulence profile of Escherichia coli O157:H7 bacteriophages isolated from sewage and livestock stools. Among 23 E. coli O157:H7 bacteriophages, 14 strains were isolated from sewage and 9 were from animal stools collected from 10 livestock farms in Korea. For each bacteriophage DNA sample, the presence of stx1, stx2, eae, aafII, ial, elt, estI, estII, astA, afa, and cnf was examined by polymerase chain reaction. The detection rate of eae, stx2, estI, astA, and ial was 100%, 69.6%, 13.0%, 13.0%, 8.7%, respectively. While all E. coli O157:H7 bacteriophages isolated from stools carried eae+stx2, stx2+eae, eae+astA, eae, stx2+eae+estI, eae+estI, stx2+eae+ial, and eae+ial were observed in bacteriophages isolated from sewage. As several plasmid-carrying virulence factors (estI, astA, and ial) were found in E. coli O157:H7 bacteriophages obtained from sewage and stools, the microbial safety of bacteriophages should be investigated in further study.
|
|
|
KEYWORD
|
|
|
Escherichia coli O157:H7, Bacteriophage, virulence factors, eae, stx2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|